On 18 August 2010 08:47, Kevin Lam <aboulia@gmail.com> wrote:

I have created a local install of galaxy and i can tell you it is not about network connection.
As I am using a local install but the 'upload' is horribly slow. a 3Gb file can't finish in 24 hours.

in the end, I used a python script to 'serve' the file via http protocol and entered a local LAN url for galaxy and it loaded up in minutes.

For your case, I think I have no issues up to 250 Mb on their galaxy main server, but as the wiki and manual (plus my experience with a local instance) suggests you would do well to put the file on a webserver and give the url instead of upload via browser.

Cheers
Kevin

On Wed, Aug 18, 2010 at 2:17 PM, asifullah khan <asifullah111@gmail.com> wrote:

Dear Officer,

I am a new user to assemble 75bp illumina solexa data. I have done single read illumina sequencing of my DNA of interest. it is a single file of about 370MB. The data, in file is initially in FASTQ format but letter the data arrangement become in some different kind of FASTq format inside the file. I want to convert the whole data in to simple FASTA format using GALAXY tools. I see the interesting videos on your site about using galaxy and it seems very interesting.
But here i fail to upload my 370MB data file as an initial step. I am using 100Mbp LANE networking. Are you people preferring any other special network connection for uploading such huge files on GALAXY.? Kindly guide in this aspect.
Moreover Kindly if possible then send me simple perl script and there command lines usage description for converting any format of FASTq in to FASTA.

Regards,
Asifullah Khan
Research officer,
DNA sequencing Labs,
ICCBS, University of Karachi, Pakistan.

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