Hi,
   I started using Galaxy on the cloud, but later I decided to install a local instance. You can check it out here http://wiki.g2.bx.psu.edu/Admin/Get%20Galaxy
    If you have a reasonable computer probably you can run gene expression analysis (tophat, cufflinks etc) locally. My local instance is way faster than the public Galaxy server. I have an I7 2600k sandy bridge overclocked to 4.2Ghz, 32 Gb DDR3 1600, GTX 570 fermi, and lots of hard drive space. You can install one of the free Linux distributions (like Ubuntu or Fedora along with Windows and have dual boot) or if you have a Mac you can also use it too. If you gonna use it only once, maybe a cloud instance is the easiest way to get it done, as Jennifer suggested.
Best,

Luciano



On Wed, Jun 6, 2012 at 9:26 AM, Jennifer Jackson <jen@bx.psu.edu> wrote:
Hi Monica,

>From your description, moving to a cloud instance is going to be your best solution:

http://usegalaxy.org/cloud

If you have questions as you are getting set up, these are best directed at the galaxy-dev@bx.psu.edu mailing list.

Take care,

Jen
Galaxy team


On 6/6/12 1:57 AM, Monica BALLARINO wrote:
Dear Galaxy team,

I'm using Galaxy Main to run paired-end RNAseq data. I'm a new user and
I'm in trouble.

I have a total of four set of data to compare: 2 negative controls and
two samples.

I uploaded already three complete histories (as fastq files) which were
around 70 Gb each (MBO11, MBO12 and MBO13). I need to upload the last
history (MBO14) but unfortunately I just started and apparently I cannot
go further 'cause I've used already 100% of my space.

Could you suggest please me a way to go on?

My login is edoardo@igbmc.fr. Let me know if you would need my password
to check my account.

Thanks a lot for your support.

Monica





___________________________________________________________
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Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using "reply all" in your mail client.  For discussion of
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  http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

  http://lists.bx.psu.edu/

--
Jennifer Jackson
http://galaxyproject.org
___________________________________________________________
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using "reply all" in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

 http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

 http://lists.bx.psu.edu/



--
Luciano Cosme

---------------------------------------------
PhD Candidate
Texas A&M Entomology
Vector Biology Research Group
www.lcosme.com
979 845 1885
cosme@tamu.edu
---------------------------------------------