Hi, I am trying to filter my fastq file with the condition of if quality score of reads is less then min score. So far, I have tried both *fastq_quality_filter* and *Filter FASTQ under NGS: QC and manipulation** *but I was not be able to do it. In the following you can see my fastq file. @F4HZV5G02CX6WP rank=0000096 x=1092.0 y=1767.0 length=45 TTGAGCAGCGGCGTCACGGCGGCGGCCTCGGCGGCCGCATAGGCG + FFFFFFFFFFFIIIIIIIIIIIIIIIIIIIIIIIIIHFFDDBDA> And these are quality scores. [37, 37, 37, 37, 37, 37, 37, 37, 37, 37, 37, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 39, 37, 37, 35, 35, 33, 35, 32, 29] I want to filter bases if their quality scores are less than 33. Any help would be greatly appreciated. -- HD