Hi,<div><br></div><div>I created a workflow (<span style="color:rgb(48,48,48);font-family:&#39;Lucida Grande&#39;,verdana,arial,helvetica,sans-serif;font-size:12px;line-height:15.600000381469727px;background-color:rgb(255,255,255)"> </span><a id="item-url" href="https://main.g2.bx.psu.edu/u/vijay-uky/w/rna-seq-analysis" target="_top" style="color:rgb(48,48,48);font-family:&#39;Lucida Grande&#39;,verdana,arial,helvetica,sans-serif;font-size:12px;line-height:15.600000381469727px;background-color:rgb(255,255,255)">https://main.g2.bx.psu.edu/u/vijay-uky/w/rna-seq-analysis</a><span style="color:rgb(48,48,48);font-family:&#39;Lucida Grande&#39;,verdana,arial,helvetica,sans-serif;font-size:12px;line-height:15.600000381469727px;background-color:rgb(255,255,255)"> )</span> and uploaded the reference genome (30 MB FASTA file) and the reads (45B FASTQ file after running the FASTQ groomer). Once I ran the workflow on the public server, it will enqueue the needed output (steps) but the job just wouldn&#39;t run. I tried to run the same on a local instance at my lab, it works fine. Was curious to know if there is some issue with running tools such as TopHat, Cufflinks and Cuffcompare.</div>
<div><br></div><div>If you could let me know, would greatly appreciate.</div><div><br></div><div>Sincerely,</div><div>Vijay</div>