---------- Forwarded message ----------
From: Zachary A Lewis <zlewis@uga.edu>
Date: Tue, Sep 13, 2011 at 3:10 PM
Subject: Re: [galaxy-user] Help with sam to bam
To: Austin Paul <austinpa@usc.edu>

Thanks Austin! That did the trick.

Zack

On Sep 13, 2011, at 4:47 PM, Austin Paul wrote:

You could try "fasta width formatter" on your reference fasta. This has helped me in the past when I received a similar error.

On Tue, Sep 13, 2011 at 11:32 AM, Zachary A Lewis <zlewis@uga.edu> wrote:

Hi,
I was wondering if someone could help me with an error message I'm getting after performing a sam to bam conversion in galaxy. I've used Bowtie to map sequence reads to a custom fasta file corresponding to one chromosome in my organism. The mapping seems to work fine, but when I attempt a sam to bam conversion, I receive the folowing error message:

An error occurred running this job: Samtools Version: 0.1.12 (r862)
Error creating indexes from reference (/galaxy/main_database/files/002/977/dataset_2977193.dat), [fai_build_core] line length exceeds 65535 in sequence 'LGVII'.
Segmentation fault

Any help would be appreciated.

Thanks,

Zack

___________________________________________________________
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org. Please keep all replies on the list by
using "reply all" in your mail client. For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

http://lists.bx.psu.edu/