Hi, I&#39;m trying to solve an issue I&#39;m having with my local installation of Galaxy (installed on my own computer, rather than on a server). I&#39;m using data in the form of fastq files from an Illumina Hi-seq and I want Galaxy to parse the bar coded sequences out into individual files for me. I&#39;ve been using the public server in the past, and I&#39;m able to use the Groomer, Joiner, Reverse-Complement, Trimmer, and Barcode Splitter tools just fine. Now I&#39;m trying to do the same thing locally on the same files. Both the large file (38 GB) and a smaller file consisting of the first 10k lines will upload just fine. However, I can only get the Groomer to work on the small file. When I use it on the large file I get an error: <span style="color:rgb(34,34,34);font-family:arial,sans-serif;font-size:12.727272033691406px;background-color:rgb(255,255,255)">&quot;Error executing tool: maximum recursion depth exceeded while calling a Python object.&quot; </span><div>
<font color="#222222" face="arial, sans-serif"><br></font></div><div><font color="#222222" face="arial, sans-serif">Any help on this would be greatly appreciated!<br clear="all"></font><div><br></div><div>- Chris M.</div>

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