Hi, I have been using galaxy succesfully for my work using its internal web-server. Recently I wanted to use it over the internet and so, I configured it with apache at /galaxy of apache's root. I followed all the instructions in setting up the proxy redirection. But I think the re-direction is not happening. I highly appreciate any suggestions to correct the issue. I checked for the mod_proxy, mod_proxy_http etc modules and they are present and switched on. Apache gives the following error in its error_log file: [Thu Aug 04 14:15:56 2011] [error] [client 127.0.0.1] File does not exist: /usr/local/apache/htdocs/galaxy Galaxy was set up in the directory /home/galaxy/galaxy-dist The lines specific for proxy setting in universe_wsgi.ini are as follows: use = egg:Paste#http port = 8080 host = localhost [filter:proxy-prefix] use = egg:PasteDeploy#prefix prefix = /galaxy [app:main] filter-with = proxy-prefix cookie_path = /galaxy use_remote_user = True remote_user_maildomain = unl.edu httpd.conf settings: RewriteEngine on <Proxy http://localhost:8080> Order deny,allow Allow from all </Proxy> <Location "/galaxy"> Order deny,allow Allow from all # Take the $REMOTE_USER environment variable and set it as a header in the proxy request. RewriteCond %{IS_SUBREQ} ^false$ RewriteCond %{LA-U:REMOTE_USER} (.+) RewriteRule . - [E=RU:%1] RequestHeader set REMOTE_USER %{RU}e RequestHeader set X-URL-SCHEME https RewriteRule ^/galaxy$ /galaxy/ [R] RewriteRule ^/galaxy/(.*) http://localhost:8080/$1 [P] # Compress all uncompressed content. SetOutputFilter DEFLATE SetEnvIfNoCase Request_URI \.(?:gif|jpe?g|png)$ no-gzip dont-vary SetEnvIfNoCase Request_URI \.(?:t?gz|zip|bz2)$ no-gzip dont-vary </Location> Thanks,