The workflow requires the input data to be in 'fastqsanger' format before being able to run. The files you uploaded from S3 are already in the correct format but this is most likely not set correctly in the metadata. So, click on the pencil icon for each of the datasets in your history and edit the data type by setting it to 'fastqsanger'. Save the changes and try running the workflow. It should work fine then.

On Mon, May 9, 2011 at 5:23 PM, Amit Indap <indapa@gmail.com> wrote:

Hi Galaxy,

I am a newbie to Amazon EC2 and have been carefully following the
steps in the screencast. I am able to upload two fastq files from the
s3 bucket:
http://s3.amazonaws.com/heteroplasmy/F4-bM4-1.fastq
http://s3.amazonaws.com/heteroplasmy/F4-bM4-2.fastq

I am also able import the published workflow
http://s3.amazonaws.com/heteroplasmy/Galaxy-Workflow-mt_analysis_0.01_strand-specific_(fastq_double).ga

But when it comes to running it, I cannot select the fastq file from
the drop down menu. I am able to view them on my GC instance, since I
imported them successfully, but am at a loss as to why
I can't select them from the drop down menu in the workflow to begin
their alignment. Is it something to do with my security group settings
in setting up the EC2 instance? Any assistance you can provide would
be great!

Thanks,
Amit

--
Amit Indap
___________________________________________________________
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org. Please keep all replies on the list by
using "reply all" in your mail client. For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

http://lists.bx.psu.edu/