Hi jen, I followed the GALAXY web cast to check the quality of RNA-seq data: one sample seem to have score above 20 in most bases (R2); but the other one is around 6-8 in most bases (R4) (see the attached PDF files).

Does this mean R4 RNA-seq data are BAD? What exactly does it mean anyway?

Thanks for your help,


-----Original Message-----
From: Jennifer Jackson [mailto:jen@bx.psu.edu]
Sent: Thu 8/18/2011 3:46 PM
To: galaxy-user@bx.psu.edu
Cc: Peng, Tao
Subject: visualization of alignment

Hello Tao,

For the Bowtie results, the aligned results may be low because the data
is RNA and not DNA. TopHat is generally considered a better choice for
RNA since it allows for bridges over splice sites (introns). The full
documentation for each program is on each tool's form and/or you can
contact the tool authors with scientific questions at

Also, a tutorial and FAQ are available here:

For visualization, an update that allows the use of a user-specified
fasta reference genome is coming out very soon. For now, you can view
annotation by creating a custom genome build, but the actual reference
will be not included. Use "Visualization -> New Track Browser" and
follow the instructions for "Is the build not listed here? Add a Custom

Help for using the tool is available here:

As stated before, please email the mailing list directly and not
individual team members. Specifically, with a "to" to the mailing list
(only) and not including team members as a "to" or "cc" unless ask to do
so when sharing private data. Our internal tracking system and public
archives rely on this method. Thank you for your future corporation.


Galaxy team

On 8/18/11 3:15 PM, Peng, Tao wrote:
> Hi jen, I have used BOWTIE to align my RNA-seq reads to HSV2 genome; out
> of 35,000,000 lines, only 621 lines left when I chose to have mapped
> reads only. How can visualize these aligned reads to HSV-2 genome?
> In the panel of converted SAM to BAM, I tried to use the data in
> trickster, but I am not sure to how to build a HSV genome as a
> reference?
> I appreciate your help,
> tao

Jennifer Jackson