Hi, I am trying to use the aaChanges tool. I have a file of SNPs in the correct interval format and a UCSC uploaded bed file of genomic intervals. These are both for the Zebrafish Zv9 build and are appropriately labeled this way in their attributes. But when I try to run the tool it says for both the SNP interval file and UCSC bed file: "Sequences are not currently available for the specified build." Am I doing something wrong with my formatting or does this tool not yet support Zebrafish Zv9 build? I tried using it in the Human Genome Variation section of the main Galaxy site as well as the Evolution section of the test site and both gave me the same error. Please let me know what is happening. Thank you so much Evan -- Evan Schwab Research Associate Megason Lab Department of Systems Biology Harvard Medical School 200 Longwood Ave Boston, MA 02115 908-938-3779