A separate point about the analysis of cufflinks data is the subject of the Pseudo Autosomal Regions in X and Y - this will make a mess of gene expression analysis in some cases especially because tophat will assign a read to both places which therefore makes it a multihit read (which you might then filter out) or it may double the true levels of reported expression. Anyone had experience/thoughts on this?

Best Wishes,

David.

__________________________________

Dr David A. Matthews

Senior Lecturer in Virology

Room E49

Department of Cellular and Molecular Medicine,

School of Medical Sciences

University Walk,

University of Bristol

Bristol.

BS8 1TD

U.K.

Tel. +44 117 3312058

Fax. +44 117 3312091