18 Jun
2013
18 Jun
'13
2:59 p.m.
Hi guys, I did quality control on my RNA-seq data using FastQC. In the report for Per base sequence quality, there are some base positions with the quality scores less than 20 ( see attached picture) . I am just wondering if there is any way to remove these? I looked at the FASTQ Quality Trimmerhttps://main.g2.bx.psu.edu/tool_runner?tool_id=fastq_quality_trimmer but am not sure if this is the right tool to use and how to use the parameter settings? Best regards Thanh