Howdy, Rich, My interpretation of the error report is that the fastq file you are trying to groom contains the indicated text (lab/solexa_public/Zon/ 111021_WICMT-SOLEXA_64KF7AAXX/QualityScore/s_3_1_sequence.txt) on a line where it expects a valid fastq header. I believe a valid header line would begin with an at sign ("@"). So perhaps somewhere along the way, your fastq file's contents were replaced by a filename. Bob H On Nov 2, 2011, at 10:09 AM, Richard Mark White wrote:
Hi, So, I am getting a fastq groomer error on some illumina data, with the following error. any ideas?
There was an error reading your input file. Your input file is likely malformed. It is suggested that you double-check your original input file for errors -- helpful information for this purpose has been provided below. However, if you think that you have encountered an actual error with this tool, please do tell us by using the bug reporting mechanism.
The reported error is: 'Invalid fastq header: lab/solexa_public/Zon/ 111021_WICMT-SOLEXA_64KF7AAXX/QualityScore/s_3_1_sequence.txt
rich
From: Jennifer Jackson <jen@bx.psu.edu> To: arabidopsis <svinekod@gmail.com> Cc: galaxy-user@lists.bx.psu.edu Sent: Wednesday, November 2, 2011 9:19 AM Subject: Re: [galaxy-user] fastq groomer
Hello Slon,
In case you are still having issues, the best use case for Illumina 1.8+ data is to run the FASTQ Groomer tool with the option "Sanger". As Peter noted, this assigns the expected datatype plus verifies content before investing time in downstream analysis.
Please let us know if more help is needed,
Best,
Jen Galaxy team
Hi all,
Fastq groomer has Solexa or Illumina 1.3+ as an input quality
asked at the sequencing facility about their machine and output and they said their format was Illumina 1.8+ (the newest). I tried to convert my fastq file into Sanger by fastq groomer, using Illumina 1.3+ as an input option and got all reads with quality of around 10... Does it mean
On 10/18/11 1:02 AM, arabidopsis wrote: format. I that
Galaxy cannot be used on a dataset with 1.8+ encoding or something else was wrong?
Thanks,
Slon
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-- Jennifer Jackson http://usegalaxy.org http://galaxyproject.org/wiki/Support ___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list:
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___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list:
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