I have a question about the groomer.  Do all Illumina runs need to be groomed, or are there situations where it can be skipped??  (My data says illumina 1.5, so ive been picking input type as illumina 1.3-1.5.)

rich



From: Jennifer Jackson <jen@bx.psu.edu>
To: Ateequr Rehman <ateeqrr@yahoo.com>
Cc: "galaxy-user@lists.bx.psu.edu" <galaxy-user@lists.bx.psu.edu>
Sent: Wednesday, February 29, 2012 10:57 AM
Subject: Re: [galaxy-user] ILLumina 1.9 Hiseq

Hello,

The input quality score type should be set as "Sanger" for your data.

Thanks!

Jen
Galaxy team

On 2/29/12 7:39 AM, Ateequr Rehman wrote:
> Dear Glaxy users and admin
>
> I ran my sequence data on FASTQC tool,
> output says it is
> Encoding Sanger / Illumina 1.9
>
> now i want to groom my file, but groomer does not have option for 1.9 in
> "Input FASTQ quality scores type"
>
> any idea which option i should select to grroom my file,
>
> later i want to run Bowtie or Tophat,
>
> Thanks
> **
>
>
>
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--
Jennifer Jackson
http://usegalaxy.org
http://galaxyproject.org/wiki/Support
___________________________________________________________
The Galaxy User list should be used for the discussion of
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