Dear Galxy admin and user

I have generated BAM file from my RNa seq data by using Bowtie with custom reference, followed by Filter SAM and SAM to BAM connversion, i wish to run cufflink, but its just givinbg me emty file,

any suggestion,
how to proceed

Ateequr Rehman
House No. 2 ground floor
Blauenstr. 10
79115 Freiburg im Breisgau