Hello Tao, It seems there has been a misunderstanding. Originally in other communications, your data was explained to be "4 samples with 2.4 GB of seq data per sample". If your 4 samples are actually a set of files with a total size of 350GB each (350GB x 4), then combined this would exceed the maximum quota of 50G per file/200G per history at the public Galaxy instance at http://usegalaxy.org. The option for this much data is to run either a local or cloud install. Help can be found at: http://getgalaxy.org http://galaxyproject.org/Admin/Cloud Questions related to local or cloud installations can be sent to the galaxy-dev@bx.psu.edu mailing list. Other users on this list may also have advice to offer about working with very large datasets. Best wishes for your project, Jen Galaxy team On 8/9/11 2:42 PM, Peng, Tao wrote:
Hi I am NEW to RNA-seq data and galaxy programs. When I try to up-load the RNA-seq data using FileZilla using my desktop PC, each file (about350 MB) takes about 1 hour to up-load.
Realizing I have 4 samples, each sample has 9 fasq.gz files, each file is about 350 GB, I really need some advice how to make this up-load work.
Thanks,
Tao
tpeng@fhcrc.org
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