Lali: In your case the workflow for capture re-sequencing should look like this: 1. QC data (groom fastq files and plot quality distribution) 2. Map the reads (use bwa) 3. Generate and filter pileup 4. Intersect pileup with coordinates of sure select bates. However, before you dive in please understand basic Galaxy functionality by taking a look at http://usegalaxy.org/galaxy101 and watching *all* Illumina-related Galaxy quickies (black boxes on the front page on Galaxy). Next, take a look at http://usegalaxy.org/heteroplasmy. Note, that we are working on bringing "industrial-strength" diploid genotyping functionality in Galaxy in the next two-three months that will include more sophisticated genotypers, recalibration and realignment tools, and novel visualization approaches. Thank for using Galaxy. anton galaxy team On Apr 5, 2011, at 2:44 AM, Lali wrote:
Hi! I am having problems with my sequencing results, but I am a newbie at this; so I am thinking there is something wrong with my analysis. So far, I've tried Galaxy and CLC Workbench, but with CLC I could not align to the whole genome, only to individual chromosomes (maybe there is a way, but by the time the trial ended I had not found it).
I used SureSelect capture kit and did single end sequencing on an Illumina. The files the lab sent me are FastQ Illumina 1.5 files, my samples were indexed, and I got a series of files each representing an Index.
What would be the standard workflow for this kind of data? Which tools/settings?
Does anyone have an example Galaxy workflow for preparing (clipping adapters, quality trimming) and mapping Targeted Resequencing Data?
Is there a way to obtain a coverage report through Galaxy?
Is it possible to ignore/discard the reads mapped when the coverage is below a certain threshold?
I know, I know, a lot of things, but I am very lost. Any help is appreciated.
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