Hello,<div>Recently i have used galaxy to find corresponding amino acids to a list of SNPs that i have. i used aaChange tool from the tool panel for this purpose. After obtaining the result i checked the correctness of this results by searching dbSNP for randomly chosen SNPs. however, some of those SNPs mapped to incorrect amino acid,</div>
<div><span class="Apple-style-span" style="font-family:arial,sans-serif;font-size:13px;border-collapse:collapse;color:rgb(34,34,34)"><div>For example,</div><div>The SNP( <span style="font-family:&#39;Lucida Grande&#39;;font-size:12px">rs11549096), was mapped by Galaxy to the change ( </span><span style="font-family:&#39;Lucida Grande&#39;;font-size:12px">Asp:Tyr/His, )</span> , Searching the dbSNP for this SNP Shows that  the Change should be  Asp :Asn and the link for this SNP in the dbSNP is :</div>
<div><a href="http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?searchType=adhoc_search&amp;type=rs&amp;rs=rs11549096" target="_blank" style="color:rgb(17,85,204)">http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?searchType=adhoc_search&amp;type=rs&amp;rs=rs11549096</a></div>
<div><br></div><div>So, the reference is correct in all cases i searched (more than a thousand), the position of change in the aa is correct, <u><b>but</b></u> the alternative aa is not correct in all cases.</div><div>What causes  this mistake, and how to solve this problem? </div>
<div>Note: my list contains hundreds of thousands of SNPs, so, i can not check each one of them.</div><div><br></div><div>prompt response is appreciated,</div><div><br></div><div>Best Regards,</div><div>Maha</div></span></div>