Inbar, as mentioned by others, the lastZ is a pairwise aligner. We have made some (very basic) wrappers for MUMmer (both nucmer and promer as well as mummerplot) which is also a (fast) multi sequence (but still pairwaise) aligner. http://mummer.sourceforge.net/ Our wrappers are very basic and simple and only intended to have a quicky access to MUMmer from galaxy. We will also post these shortly on the tools sections. After testing cycles have been added. For beta testing, if you have interest, drop me a pm. Cheers Alex ________________________________ Van: galaxy-user-bounces@lists.bx.psu.edu [galaxy-user-bounces@lists.bx.psu.edu] namens Inbar Plaschkes [inbar.plaschkes@gmail.com] Verzonden: woensdag 13 oktober 2010 11:41 Aan: galaxy-user@bx.psu.edu Onderwerp: [galaxy-user] LastZ on galaxy Dear galaxy team, I would like to know whether it is possible to perform LastZ (multiple sequence alignment tool for aligning different genomes to each other) ? if so then how ? is there a tutorial you can refer me to ? many thanks Inbar -- ______________________________________________________________ Inbar Plaschkes Bioinformatics Core Facility National Institute for Biotechnology in the Negev Building 51, room 314 Ben-Gurion University of the Negev Beer-Sheva 84105, Israel Email: inbar.plaschkes@gmail.com<mailto:inbar.plaschkes@gmail.com> Tel: 08-6479034 054-7915931 Fax: 08-6472983 http://bioinfo.bgu.ac.il