I am a new user to Galaxy, and I was hoping someone might be able to help with this small problem.  I am using Drosophila resequencing data downloaded from the SRA and trying to look for SNPs that differentiate the two strains.  I&#39;ve gotten as far as generating the pileup for each strain, but my column which has the consensus base is always an N.  Can anyone help me out?<div>


<br></div><div>thanks, Christine<br clear="all"><br>-- <br>Christine J. Picard, Ph.D.<br>Postdoctoral Research Associate<div>Department of Entomology</div><div>Texas A&amp;M University</div><div>TAMU 2475</div><div>College Station, TX </div>


<div>77843-2475</div><div><a href="mailto:christine.picard@gmail.com" target="_blank">christine.picard@gmail.com</a></div><br>
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