Dear Sir or Madam, Recently I was trying to use Galaxy for my data analysis but ran into problems..... Condition: I used galaxy server at PSU. I tried load local .bam file directly to galaxy, I also tried FTP, but both ways resulted into the same outcome. The .bam files I was using are pure .bam files with no index. Problems: 1.After I load them, the file was recognized as .txt file, and it is "empty" (But it did take long time to upload to FTP ~1GB), in the annotation window, it shows "The uploaded binary file contains inappropriate content" 2. I tried to convert the file to .bam format, it returns "An error occurred setting the metadata for this dataset. You may be able to set it manually or retry auto-detection." Could you help me for this, I just uploaded a small file to the FTP, just in case you need it for a test. Username: ylc35@mail.missouri.edu Great thanks~ YL