Hello,

I am attempting to megablast a FASTA file containing ~45,000 records of 100nt each against the nt database using the Galaxy web interface. I am using a Galaxy-generated FASTA file produced from a groomed FASTQ file using the 'FASTQ to tabular' and 'Tabular to FASTA' tools. When I try to use this as input for the Megablast tool, I get the "Required metadata values are missing" error. When I look at the attributes of the FASTA file, the datatype is indeed set to FASTA. I have tried downloading the FASTA file and reuploading it as a FASTA file explicitly with the same result. I also tried changing the FASTA width to 79, and trimming the records to 70nt each – not the problem. If I make a file containing only the first FASTA record, I get the same error. Any suggestions?

Thanks!

Elaine