Hi,

       I am a first time user in galaxy for analysis of my RNA-Seq data.

I am facing problem in loading data in galaxy.

1.I successfully transfered fastq files (from Illumina) by FTP and then tried to upload by checking each files and execute.

3. Initially it was yellow (with rotating circle) and then turned pink instead of green. It seems probably there is an error in uploading. But the files disappeared from the upload page but lined up with pink (at the history) and shows that 72.4GB of the disk space are occupied. I can view the files also with ‘display data with viewer’!

4.when I tried analyze with TOPHAT, it tells you have no files in history!

5. When I wanted to do the whole process again but FTP tells yours files are already exists (transferred) in the server. But since they are not at the upload page, I can not upload again each one by checking them to correct the error?

6. Could you please advice me how to solve this problem and successfully upload files that would be good for analysis.

Thanks for your help,

Amit K. Maiti, PhD

Oklahoma medical Research foundation,

Oklahoma city, ok 73104

Phone-405 271 8099

Mobile-409 256 9557