Hi, Nucleotide distribution is much easier to read when it is drawn as lines : http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc/good_sequence_short_fa... instead of bars : http://main.g2.bx.psu.edu/static/fastx_icons/fastq_nucleotides_distribution_... Is there a way to produce a line graph in http://main.g2.bx.psu.edu/ ? Cheers Christophe -- ==================================================================== = Christophe KLOPP BIA INRA Toulouse 31326 Castanet-Tolosan = = Tel: 33 5 61 28 50 36 Email: christophe.klopp@toulouse.inra.fr = = http://www.sigenae.org/ = = http://bioinfo.genotoul.fr/ = ====================================================================