Hi Sujata- Are you using a local instance, a cloud instance, or Galaxy Main? Also, could you tell me where in the instructions at http://wiki.g2.bx.psu.edu/FTPUpload you're having problems? That will help folks figure out where you're having problems. (Keep in mind that an FTP 421 error can mean a lot of things.) -Scott ----- Original Message ----- Hello, I am trying to upload 27.2 GB fastq file in Galaxy through FTP, but it throws an error: netout: Connection reset by peer 421 Service not available,remote server has closed connection. Please help. Thank you, Sujata ___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/