Hi, I&#39;ve been using lastz to map some 454 reads to a reference sequence on galaxy.  I&#39;m not clear on what the mapping modes do for the analysis.  If I am already setting a cut-off value for the percent identity for reporting matches then what exactly does the mode setting do? In the tutorial you set a roche-454 98% mode based for a re-sequencing example and then set the identity to 90%, what parameters in lastz does the mapping mode change?<br>
<br>thanks, <br>H<br clear="all"><br>-- <br>Heather Kent<br>Bioinformatics Core Facility<br>Public Health Agency of Canada<br>National Microbiology Laboratories<br>(204)784-7503<br><br>Quote of the week:<br>Most people are other people. Their thoughts are someone else&#39;s opinions, their lives a mimicry, their passions a quotation.<br>
    Oscar Wilde (1854 - 1900), De Profundis, 1905<br>