Actually I was running blast separately and got error messages from them as well and I assumed it was a connection problem But in fact it was two separate problems unrelated. Good catch And thanks again

Vermont Cancer Center
Advanced Genome Techology Lab

On April 28, 2014 4:28:49 PM EDT, Peter Cock <> wrote:
On Mon, Apr 28, 2014 at 9:01 PM, Scott Tighe <> wrote:

Thank you for your detailed response and I should have noted that I was
using the Main Public Galaxy. Thank you for confirming that it is down!

I appreciate your input!


Hi Scott,

Galaxy is back for me now, and yes, the Galaxy tool you are
talking about is called Megablast (version 1.2.0), and only
offers these target databases:

htgs 13-Apr-2014
nt 17-Apr-2014
wgs 20-Apr-2014

This Galaxy tool does *not* connect to the NCBI BLAST
service over the internet.

It sounds like the problem was due to the Galaxy team
updating these databases - as Jennifer mentioned?

I don't understand how you! got an NCBI web server
error message (in your email to Wayne), but perhaps
you were separately testing the NCBI BLAST service
outside of Galaxy?