Dear Galaxy When running HiSeq shot metagenomics sample from the environment against megablast and taxonomic representation, How do I filter/remove all the 16s and other conserved sequences. The problem if blasting a single organism that has a fraction of conserved sequence, the results will align with E.coli 10,000 times more then the possible target organism. This data would be wrong and misleading. For example a 100mg sample that was negative for e coli using MUG test, give thousands of hits with galaxy. 1) Is there a "filter conserved sequences" setting? 2) Is there a "remove model organisms" setting? Scott Tighe -- Core Laboratory Research Staff Advanced Genome Technologies Core Deep Sequencing (MPS) Facility Vermont Cancer Center 149 Beaumont Ave University of Vermont HSRF 303 Burlington Vermont USA 05045 802-656-AGTC 802-999-6666 (cell) Quoting Jennifer Jackson <jen@bx.psu.edu>:
Hello Elwood,
Are you still having connection issues today? Or is this resolved?
Best,
Jen Galaxy team
On 9/13/13 11:36 AM, Elwood Linney wrote:
A message sent earlier this week by me indicated that I could not connect to Galaxy via Fetch to download data.
A reply indicated a glitch was fixed.
I then could connect with Fetch and I tried to transfer 4 x 16gb files and the connection disconnected about 4 times.
Now, once again, I cannot connect with Galaxy online to transfer data.
Is this a problem that can be solved-either at my end or at Galaxy?
Elwood Linney
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