Thanks again  Jen,

I just would like to get this point: if I want to modify them then I won’t be using Galaxy right, since there are few of them I can modify.

All I would like to know is: are the hidden/chosen/not accessible parameters CHOSEN by Galaxy? If yes, is there any chance to know what has been chosen for these parameters which are not visible? Or did I understand that are default as written in the manual?

Hope it’s clear, sorry for insisting but I need to know as I am doing an analysis for someone else and have to report all the options I have opted for! I cannot tell anything about those which are not listed

I have read the link you sent me , but I cannot find all the missing options…I opened all the links on it an found only those already listed in the main cufflinks running page(see attachment)..

Thanks again for your patience,

Best,

Irene

From: Jennifer Jackson [mailto:jen@bx.psu.edu]
Sent: 15 November 2013 20:59
To: Irene Bassano; 'galaxy-user@bx.psu.edu'
Subject: Re: [galaxy-user] FW: Tophat/Cuff parameters

Hello -

Most of these are at defaults. The source code is the best place to find any that are hard-coded (if included, most likely done to manage resources). Again, any of of this could be modified if you have programming resource. The repository is owned by the devteam and in the Tool Shed, search with "cuff" here: http://usegalaxy.org/toolshed

Best,
Jen
Galaxy team

On 11/15/13 2:59 AM, Irene Bassano wrote:

Hi Jen,

Thanks again.

Just to make it clear: if all the options are not present on galaxy but only some of them, does this mean that the absent ones have been set by galaxy dev? are these to be considered best settings not worth changing? I would like to learn a bit more and “play” with all options but if some of them wont affect the final reaults then is not worth changing them.

Thanks,

Irene

From: Jennifer Jackson [mailto:jen@bx.psu.edu]
Sent: 14 November 2013 21:43
To: galaxy-user
Cc: Irene Bassano
Subject: Tophat/Cuff parameters

Hello,

Yes, this is correct, not all parameters can be adjusted in the UI. Many can, especially with Tophat, but not all. The list of implemented parameters that will match the documentation is listed at the bottom of each of these tools. The UI form itself up top may have a slightly different human readable name, but most will include the parameter name "-r", etc.

To see all of the parameters on the forms:
1. Tophat/2 - open "TopHat settings to use: Full parameter list"
2. Cufflinks/merge/compare/diff - open or modify any listed on form to see full content

This tool is in the Tool Shed (http://usegalaxy.org/toolshed), so if you had programming resource available, then these could certainly be modified. Development questions can go do the galaxy-dev@bx.psu.edu mailing list, but really the best place to start is in our wiki:
http://wiki.galaxyproject.org/Admin/Tools/ToolConfigSyntax
http://wiki.galaxyproject.org/Tool%20Shed

It would be really helpful if you sent questions directly to the galaxy-user@bx.psu.edu mailing list going forward.
Thanks,

Jen
Galaxy team

On 11/14/13 1:08 PM, Irene Bassano wrote:
> Hi Jen,
> thanks a lot for your reply.
>
> I am trying to apply some changes to certain parameters in cufflinks. I have read them on the manual but on galaxy are not all listed.
> is this because they have been chosen already by galaxy and set to certain values you guys believe are optimal?
> I cannot find for example:
>
> Maximum genomic length allowed for a given bundle.
> Minimum intron size allowed in genome.
> Minimum average coverage required to attempt 3' trimming.
>
> and many others.
>
> I also wanted to access full parameters for Tophat and Cuffdiff...
>
> Any suggestion on how can I access them?
>
> Thanks,
> Irene
--
Jennifer Hillman-Jackson
http://galaxyproject.org

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