Hi Franzi, You have one too many hg19 in there. The fields go like: <unique_build_id> <dbkey> <display_name> <fasta_file_path> <valid_tool_suites> so: hg19 hg19 hg19 /drive1/galaxy/reference/hg19/sam_index/hg19_ref.fa gatk But do note that these tool integrations are still undergoing active development. Please report bugs if you encounter any. Thanks for using Galaxy, Dan On Nov 16, 2011, at 6:29 PM, Metge, Franziska wrote:
Dear happy users of Galaxy,
We are running Galaxy locally. It's a very fine tool! By now everything works fine, except when I try to run any GATK program. I usually get this error message:
##### ERROR ------------------------------------------------------------------------------------------ ##### ERROR A USER ERROR has occurred (version 1.3-14-g348f2db): ##### ERROR The invalid arguments or inputs must be corrected before the GATK can proceed ##### ERROR Please do not post this error to the GATK forum ##### ERROR ##### ERROR See the documentation (rerun with -h) for this tool to view allowable command-line arguments. ##### ERROR Visit our wiki for extensive documentation http://www.broadinstitute.org/gsa/wiki ##### ERROR Visit our forum to view answers to commonly asked questions http://getsatisfaction.com/gsa ##### ERROR ##### ERROR MESSAGE: The fasta file you specified (/tmp/tmpp0oxJu/hg19) does not exist. ##### ERROR ------------------------------------------------------------------------------------------
my picard_index.loc line for the hg19 reference looks like this:
hg19 hg19 hg19 hg19 /drive1/galaxy/reference/hg19/sam_index/hg19_ref.fa gatk
also the bam file I am submitting to GATK has the reference genome specified in it's attributes.
Could please anyone help me. Thank you Franzi
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