Hi galaxy dev team:

 

I just installed galaxy on my ubuntu box and tried to do RNA-seq analysis according to Jeremy's excellent tutorial

 

http://main.g2.bx.psu.edu/u/jeremy/p/galaxy-rna-seq-analysis-exercise

 

however, I encountered following error messages when I was trying to execute Cufflinks in galaxy..

 

Dataset generation errors

Dataset 121: Cufflinks on data 92: gene expression

Tool execution generated the following error message:

Error running cufflinks. [Errno 2] No such file or directory: 'transcripts.gtf'

The tool produced the following additional output:

cufflinks v1.0.3

cufflinks -q --no-update-check -I 300000 -F 0.050000 -j 0.050000 -p 4 -b

 

how should I solve this problem?

 

Thanks in advance.

 

 

Best Regards,

 

John Wu

 

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