Hi all,<br>We are just beginning to analyze RNA-seq data and hope to make use of the Galaxy tools. We are not using a commonly studied organism such as human. Rather, our data are from the bacterium Pseudomonas fluorescens Pf0-1.<br>
<br>The genome for P. fluorescens Pf0-1 appears in the &quot;database/build&quot; menu, and we have selected this in the .sam file attributes.<br><br>When we run the sam to bam conversion, we get the error &quot;<span style="vertical-align: middle;">Sequences are not currently available for the specified build.&quot;<br>
<br>Is this something that can be corrected easily?<br><br>Many thanks for your help.<br><br>Mark Silby<br></span>