Dear Galaxy

for genome wide metagenomics, I wonder if there is a way to purge conserved sequences. For example, if I have 99% of 10 million reads have perfect alignment  to more then 20 reference sequences, I want them to go away. Inotherwords I only want unique sequences that align to only a few reference sequences. As for 16sDNA, a 100bp fragment taken from between V5 and V6 will align to everything!!! I want to purge that away

Sincerely

Scott
Scott Tighe
Advanced Genome Technology Lab
Vermont Cancer Center at the University of Vermont
149 Beaumont Avenue
Health Science Research Bd RM 305
Burlington Vermont USA 05405
lab  802-656-AGTC (2482)
cell 802-999-6666

On 2/28/2012 6:19 PM, Jennifer Jackson wrote:
Hi,

You will want to assign yourself as an administrator in the universe_wsgi.ini file.

# Administrative users - set this to a comma-separated list of valid Galaxy
# users (email addresses).  These users will have access to the Admin section
# of the server, and will have access to create users, groups, roles,
# libraries, and more.  For more information, see:
# http://wiki.g2.bx.psu.edu/Admin/Interface
#admin_users = None
^^ uncomment and add your login email here

Other wikis that may be helpful, especially if user login is not yet set up:
http://wiki.g2.bx.psu.edu/Admin/Config/Performance/Production%20Server
http://wiki.g2.bx.psu.edu/Admin/Config

Hopefully this helps,

Jen
Galaxy team

On 2/28/12 2:59 PM, Alejandra Rougon wrote:

Thank you Greg, I saw the wiki but I do not see the admin link in my
galaxy interface. How do you open galaxy as an administrator?
t
------------------------------------------------------------------------
*From:* Greg Von Kuster <greg@bx.psu.edu>
*To:* Alejandra Rougon <alerougon@yahoo.com>
*Cc:* "galaxy-user@lists.bx.psu.edu" <galaxy-user@lists.bx.psu.edu>
*Sent:* Tuesday, 28 February 2012, 14:02
*Subject:* Re: [galaxy-user] Speed up uploading into local Galaxy,
terribly slow!!

You can upload your large file to Galaxy data libraries using a
combination of "Upload files from filesystem paths" and "Do not copy
data into Galaxy's default file store".

See this wiki:

http://wiki.g2.bx.psu.edu/Admin/Data%20Libraries/Uploading%20Library%20Files

For all of the information on Galaxy data libraries, see this wiki:

http://wiki.g2.bx.psu.edu/Admin/Data%20Libraries/Libraries

Greg Von Kuster


On Feb 28, 2012, at 2:54 PM, Alejandra Rougon wrote:

Hello, I tried to search in the forums and although this question has
appeared many times I still don't have a solution.

I cannot manage to upload big files into the local galaxy, it just
takes ages. Can I not just copy and paste into a local directory? why
do I have to upload the files if it is already installed locally?
I do not have a server webpage in order to use the url address option
If I do it through ftp (locally) what ftp address shall I put? ftp
localhost:8080??

Is there any other option to speed up uploading, is so slow that is no
longer worth using it, please help me!

___________________________________________________________
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org <http://usegalaxy.org>. Please keep all replies on
the list by
using "reply all" in your mail client. For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

http://lists.bx.psu.edu/





___________________________________________________________
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using "reply all" in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

   http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

   http://lists.bx.psu.edu/