Hello Tomaž,

There are three trimming tools. One is from the FASTX Tool kit, the others are not, and all can be run from the command line. They can be found in the Galaxy distribution:
http://getgalaxy.org

Tool names that you will see in the web browser mapped to where the wrappers are in the distribution. The xml file is the form displayed, the py file is the actual script. The 2nd one is the tool you asked about and it is not from the FASTX Toolkit.


FASTQ Trimmer

   galaxy-dist / tools / fastq / fastq_trimmer.xml
   galaxy-dist / tools / fastq / fastq_trimmer.py


FASTQ Quality Trimmer ( by sliding window)

   galaxy-dist / tools / fastq / fastq_trimmer_by_quality.xml
   galaxy-dist / tools / fastq / fastq_trimmer_by_quality.py


Trim sequences

   galaxy-dist / tools / fastx_toolkit / fastx_trimmer.xml

FastXToolkit source: http://hannonlab.cshl.edu/fastx_toolkit/


Thanks!

Jen
Galaxy team

On 4/17/13 4:30 AM, Tomaz Rijavec wrote:
Dear "Galaxy Team",

I have a question regarding the fastq quality trimmer (by sliding window) tool. What is the script running behind it? Is it a modified version of the fastx_quality_trimmer found in the fastx tools package? Is there a standalone version of it one can set up locally and run it from the command line.
Thanks for all the info you can provide.

best regards,
Tomaž





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dr. Tomaž Rijavec
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Institute of Physical Biology / Toplarniška ulica 19, SI-1000 Ljubljana, Slovenia / T: +3861 587 54 70 / www.ifb.si


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