Hi Andrew,

My first guess is that perhaps the region is not set correctly?
http://wiki.galaxyproject.org/CloudMan/AWS/GettingStarted

See " Step 1: One Time Amazon Setup", subsection 2, where region is " set your AWS Region to US East (Virginia)".
The image in the wiki for step 1.2 is slightly outdated, instead it will look like this:

Please give this a try and let us know if you continue to have issues.

Thanks!

Jen
Galaxy team

On 4/8/13 3:56 PM, Thompson, Andrew wrote:

Dear Jen
Thanks. I have merged the files and end up with 4 x 47 G fastq files for read mapping to the reference.
It seems this is too much data to analyse on the public main instance if the size limit is 250 G?
So I tried to set up the cloud option following the screencast (http://screencast.g2.bx.psu.edu/cloud/), but when I search for the current AMI name (861460482541/galaxy-cloudman-2011-03-22) it is not found in the list of community AMIs under Amazon's EC2 Management Console. Any ideas why this is not working?
regards
   Andrew

-- 
Jennifer Hillman-Jackson
Galaxy Support and Training
http://galaxyproject.org