Hi, I run Barcode Splitter (from public Galaxy server) and am unable to download the text files generated by the barcode splitter, got an error message which says ' Could not find 'FASTQ_Groomer_on_data_1__V1.txt' on the extra files path /galaxy/main_pool/pool2/files/003/610/dataset_3610002_files/FASTQ_Groomer_on_data_1__V1.txt. The run was Ok (green colour) but yet i was unable to download the text files. Could you please advise? Also, I wonder if you could help regarding the local Galaxy.. I used VirtualBox to install the local copy, and installed according to the instructions as in the website, command as "hg clone https://...." The setup was smooth and went OK. The problem starts when I run the local Galaxy using real data. I could upload the files, but have problems with the fastQC and barcode splitter, I could not proceed any further since these two operations could not be run...The error messages are: FastQC An error occurred running this job: Traceback (most recent call last): File "/home/Desktop/galaxy-dist/tools/rgenetics/rgFastQC.py", line 141, in <module> assert os.path.isfile(opts.executable),'##rgFastQC.py error - cannot find executable %s' % opts.executable AssertionError Barcode Splitter An error occurred running this job: /home/Desktop/galaxy-dist/tools/fastx_toolkit/fastx_barcode_splitter_galaxy_wrapper.sh: line 65: fastx_barcode_splitter.pl: command not found gzip: stdout: Broken pipe Can anyone help me please? I installed the local Galaxy but could not proceed further. I then used the public Galaxy but could not download the files.... Hope to hear from you. Thanks very much. Best regards Alicia