Hi,
I just notice that NGS FASTQ Trimmer by column can't detect the fastq file I loaded. Anyone knows why. thanks a lot.


On Fri, Jan 24, 2014 at 11:00 AM, <galaxy-user-request@lists.bx.psu.edu> wrote:
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Today's Topics:

   1. Re: Creating a Trackster visualisation from a     reference in
      your history (Jeremy Goecks)


----------------------------------------------------------------------

Message: 1
Date: Thu, 23 Jan 2014 15:49:44 -0500
From: Jeremy Goecks <jgoecks@email.gwu.edu>
To: graham etherington (TSL)
        <graham.etherington@sainsbury-laboratory.ac.uk>
Cc: galaxy-user@lists.bx.psu.edu
Subject: Re: [galaxy-user] Creating a Trackster visualisation from a
        reference in your history
Message-ID: <C3382F38-D243-4979-B6A7-BA13EF161EE5@email.gwu.edu>
Content-Type: text/plain; charset="windows-1252"

> Is it possible to create a custom build and use it to view a SAM file without adding the .len and .2bit files in to the Galaxy file system as an administrator?

Yes, it definitely is.

> If so, what am I doing wrong?


This is a Galaxy bug which has been fixed in this commit:

https://bitbucket.org/galaxy/galaxy-central/commits/117fef56513fc563dd231516196cfd601c1635e2

We have a release coming up, so this fix will be included in the release and will make it to our public server soon. In the meantime, note that you can use the genome fasta file rather than the len file to create a custom build and everything should work.

Thanks,
J.

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--
Gang Wang
Ph.D. student
Veterinary Integrative Bioscience
College of Veterinary Medicine & Biomedical Sciences
Texas A&M University
College Station, TX  77843-4458