to the best of my knowledge reads at the start of SOLiD data set also have a higher error rate ..
On Wed, Nov 9, 2011 at 12:03 PM, Bob Harris <rsharris@bx.psu.edu> wrote:Yes, reads at the start and the end of the file come from the edge of
> David, in my experience with Illumina sequencing, it looks like the reads at
> the start of a file have a much higher sequencing error rate.
> Bob H
the Illumina slide, and tend to be of poorer quality that the reads from
the middle.