Hi Delong, I used Tophat2 for 32 files and all worked well. I did not have any problem with it. Best wishes, Anto ________________________________ From: galaxy-user-bounces@lists.bx.psu.edu [galaxy-user-bounces@lists.bx.psu.edu] on behalf of Delong, Zhou [Delong.Zhou@USherbrooke.ca] Sent: 29 August 2013 14:57 To: galaxy-user@bx.psu.edu Subject: [galaxy-user] Tophat2 not working? Hello, I've been trying tophat 2 on the public server since last week but I don't get any results. I got one empty output for " the output datasets were deleted while the job was running, the job was manually dequeued or there was a cluster error." one error with " Unable to queue job for execution. Resubmitting the job may succeed." and one empty output with no error message. It seems that tophat 2 is new on the server, has anyone run it with success yet? Thanks, Delong