I just installed a new instance of Galaxy and
bwa_wrappers from the main toolshed. I then uploaded a
reference genome along with two FASTQ files in order to run "Map
with BWA for Illumina". When I run it, I get this error:
The alignment failed.
Error aligning
sequence. [bwa_aln] 17bp reads: max_diff = 2
[bwa_aln] 38bp reads:
max_diff = 3
[bwa_aln] 64bp reads:
max_diff = 4
[bwa_aln] 93bp reads:
max_diff = 5
[bwa_aln] 124bp
reads: max_diff = 6
[bwa_aln] 157bp
reads: max_diff = 7
[bwa_aln] 190bp
reads: max_diff = 8
[bwa_aln] 225bp
reads: max_diff = 9
[bwt_restore_bwt]
fail to open file
'/export/galaxy-dist/database/files/000/dataset_1.dat.bwt'.
Abort!
Aborted
I've used the same tool on other Galaxy instances
and it always auto-indexes the reference genome here before
continuing. What's going wrong? (I made sure bwa was in the
user's PATH that is running run.sh)