What does it tell you about the number of N's (uncalled bases)?


From: "Tan, Justin" <jtan@fas.harvard.edu>
Date: Thu, 28 Mar 2013 10:41:24 -0400
To: "galaxy-user@lists.bx.psu.edu" <galaxy-user@lists.bx.psu.edu>
Subject: [galaxy-user] FastQC Issue with per base nucleotide quality graph

Hi,

I am having a problem with FastQC. When I view per base quality, it gives me a strange looking graph:


I am wondering it this is because of a problem with my data? None of my colleagues have seen this before.

Thanks!
Justin



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