Hello! I use Galaxy to convert unaligned .bam files (RNA-seq) to fastq files. That works! Then i want to align the fastq files using TopHat2 or Tophat, but the analysis does not start since yesterday. However, I received the following error message several times since yesterday: Details user username null quota_percent 30 total_disk_usage 81718693796 nice_total_disk_usage 76.1 GB email gerwin.heller@meduniwien.ac.at tags_used model_class User id 12477bb953a54e27 source HDACollection(9b2b9157fa89d388,193) xhr readyState 4 responseText "Error in history API at listing contents: url_for could not generate URL. Called with args: ('history_content',) {'history_id': u'9b2b9157fa89d388', 'id': 'bbd44e69cb8906b5a211f8cf18ab3f22'}" responseJSON Error in history API at listing contents: url_for could not generate URL. Called with args: ('history_content',) {'history_id': u'9b2b9157fa89d388', 'id': 'bbd44e69cb8906b5a211f8cf18ab3f22'} status 500 statusText Internal Server Error responseHeaders Server nginx/1.4.4 Date Tue, 04 Mar 2014 12:52:02 GMT Content-Type application/json Transfer-Encoding chunked Connection keep-alive Cache-Control max-age=0,no-cache,no-store options data parse true emulateHTTP false emulateJSON false Thanks for help!! With kind regards, Gerwin Heller -- Gerwin Heller, Ph.D. Medical University of Vienna Department of Medicine I Division of Oncology Währinger Gürtel 18-20 A-1090 Vienna, Austria Phone: +43 1 40400 4433 Fax: +43 1 40400 4451