I have had the same problem, and am also a newbie to NGS with Illumina. The work-around I found was to download bowtie directly from the website, run it on your local computer, then upload the resulting SAM file for subsequent Galaxy-driven analysis. Not optimal, I know, but if you are in a hurry...
Best,
Dan
Hi all,I'm new to next-gen sequencing, so please be gentle. I've just received a pair of Illumina FASTQ files from the sequencing facility and intend to map them to the hg19 reference genome. I first used the FASTQ Groomer utility to convert the reads into Sanger reads. However, when running Bowtie for Illumina on the resulting dataset under default settings, I received the following error:An error occurred running this job: Error aligning sequence. requested number of bytes is more than a Python string can holdCan someone help point out my mistake? My history is accessible at http://main.g2.bx.psu.edu/u/wengkhong_lim/h/chip-seq-pilot-batchAppreciate the help!Weng Khong, LIMDepartment of Genetics
University of Cambridge
E-mail: wkl24@cam.ac.uk
Tel: +447503225832
_______________________________________________
galaxy-user mailing list
galaxy-user@lists.bx.psu.edu
http://lists.bx.psu.edu/listinfo/galaxy-user