Dear Jen, after a couple of days of running cufflinks on a sam file (data 9) with an annotated reference in gtf (data 28), I have this message in return that I am not able to decode: 32: Cufflinks on data 9 and data 28: total map mass error An error occurred running this job:cufflinks v2.0.2 cufflinks -q --no-update-check -I 300000 -F 0.050000 -j 0.150000 -p 8 -G /galaxy/main_pool/pool6/files/005/663/dataset_5663874.dat -u -N -b /galaxy/data/hg19/sam_index/hg19.fa Error running cufflinks. return code = 1 cufflinks: /lib64/lib Thank you for you help Best regards Davide --- Davide Degli Esposti, PhD Epigenetic (EGE) Group International Agency for Research on Cancer Tel. +33 4 72738036 Fax. +33 4 72738322 150, cours Albert Thomas 69372 Lyon Cedex 08 France ----------------------------------------------------------------------- This message and its attachments are strictly confidential. If you are not the intended recipient of this message, please immediately notify the sender and delete it. Since its integrity cannot be guaranteed, its content cannot involve the sender's responsibility. Any misuse, any disclosure or publication of its content, either whole or partial, is prohibited, exception made of formally approved use -----------------------------------------------------------------------