Hi Haluk,
By filtering, you mean removing reads? trimming their ends? or masking some of their bases?
There are 3 tools under "Generic FASTQ manipulation" that may help you:
    Filter FASTQ reads by quality score and length
    FASTQ Quality Trimmer by sliding window
    FASTQ Masker by quality score
Regards,
Florent

On 31/07/11 05:58, Haluk Dogan wrote:

Hi,

I am trying to filter my fastq file with the condition of if quality score of reads is less then min score.

So far, I have tried both *fastq_quality_filter* and *Filter FASTQ under  NGS: QC and manipulation* but I was not be able to do it.

In the following you can see my fastq file.

@F4HZV5G02CX6WP rank=0000096 x=1092.0 y=1767.0 length=45

TTGAGCAGCGGCGTCACGGCGGCGGCCTCGGCGGCCGCATAGGCG

+

FFFFFFFFFFFIIIIIIIIIIIIIIIIIIIIIIIIIHFFDDBDA>

And these are quality scores.

[37, 37, 37, 37, 37, 37, 37, 37, 37, 37, 37, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 40, 39, 37, 37, 35, 35, 33, 35, 32, 29]

I want to filter bases if their quality scores are less than 33.

Any help would be greatly appreciated.

--
HD

___________________________________________________________
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using "reply all" in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

  http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

  http://lists.bx.psu.edu/