Dear Galaxy team,

I just discovered your tool and is fantastic!. I want to reannotate the porcine affymetrix microarray. So I wanted to perform the following steps:

1. Run blat/blast to the porcine genome SGSC sscrofa9.2/susScr2 with the sequences of all probes on the array (which i have in a csv file)
2. Get genes
3. Do the same with human genome

I can load my file and the entire pig genome but i do not know how to perform the “alignment”

Is it possible to perform blat/blast (or any other alignment tool similar) through Galaxy?

Thanks very much in advance,

Lara Nonell

Microarray Analysis Service

Scientific and Technical Services

IMIM-Hospital del Mar

Barcelona Biomedical Research Park (office 166)

Doctor Aiguader, 88 | 08003 Barcelona

Tel.+34 933 160 577 | Fax +34 933 160 410

lnonell@imim.es

www.imim.es