Recently I am using cufflinks to analyze differential expression between different conditions, but when using cufflinks an error occurred:
An error occurred running this job:cufflinks v1.0.3
cufflinks -q --no-update-check -I 1 -F 0.050000 -j 0.050000 -p 8 -g /galaxy/main_database/files/002/991/dataset_2991920.dat
Error running cufflinks. [bam_header_read] EOF marker is absent.
[bam_header_read] invalid BAM binary header (this
However, when I used the same parameters to analyze another file, it worked well:
19,904 lines
format: gtf, database: rhodRHA1
Info: cufflinks v1.0.3
cufflinks -q --no-update-check -I 1 -F 0.050000 -j 0.050000 -p 8 -g /galaxy/main_database/files/002/991/dataset_2991920.dat
The only difference is the size of each file, the failed one input file is 23 G, while the succeeded one input file is 3.5 G, is the size causing failure?
Thank you in advance.
Best wishes!
Sincerely, Jinhai YU
010-64888521
Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing, 100101, China