Dear Galaxy Officer,

Good day.

I am a new user of Galaxy main server. The tools provided are very user-friendly. Thanks for the establishment of these.

I just new to the RNA-seq analysis and now in the learning process of Bioinformatics.

I would like to inquire on the FastaQC report generated on my data.

For your information:

Samples: Plant (dicotyledon)

Type of data: RNA-seq (Illumina HiSeq 2000 with CASAVA v 1.8.2)

Paired ends

Adapter sequence: RPI 15 ( 5’ CAAGCAGAAGACGGCATACGAGATTGACATGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA)

Main purpose of my analysis: Identification of novel transcript and gene expression studies

I run FastQC on my raw RNA-seq data both forward and reverse. I attach the FastQC report in this email.