FastQCFastQC Report
Mon 22 Jul 2013
D1TB3ACXX_PR0055_02A15_H1_L007_R1.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename D1TB3ACXX_PR0055_02A15_H1_L007_R1.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 50140881
Filtered Sequences 0
Sequence length 100
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTCCA 9595585 3.510654 6.3710628 60-64
TTCTC 12116685 3.2885883 5.2676396 45-49
TCTCG 10716155 3.2443743 5.334085 80-84
CCAAG 11237210 3.0798802 5.2831144 55-59
CTTCT 11243660 3.0516405 5.6570544 95-96
GCCAA 11103775 3.0433087 5.249829 55-59
TCTTC 10632210 2.8856869 5.412275 90-94
TGCCA 9846895 2.8365011 5.120373 50-54
CTGCT 9346185 2.8296084 6.1322436 95-96
ACTCC 7230155 2.6452348 5.5269346 60-64
AAAAA 24620425 2.5958595 8.515192 95-96
CCGTC 5590090 2.3977835 6.3717356 85-89
GTGCC 7063720 2.3855677 5.072632 50-54
TCTGC 7538220 2.282237 5.32954 95-96

Files created by FastQC

D1TB3ACXX_PR0055_02A15_H1_L007_R1_fastqc.zip (171.6 KB)
duplication_levels.png (18.5 KB)
fastqc_data.txt (7.8 KB)
fastqc_report.html (8.0 KB)
kmer_profiles.png (65.5 KB)
per_base_gc_content.png (10.2 KB)
per_base_n_content.png (7.7 KB)
per_base_quality.png (9.8 KB)
per_base_sequence_content.png (16.5 KB)
per_sequence_gc_content.png (26.7 KB)
per_sequence_quality.png (19.4 KB)
rgFastQC3Jm48N.log (1.7 KB)
sequence_length_distribution.png (17.2 KB)
summary.txt (751 B)
FastQC documentation and full attribution is here

FastQC was run by Galaxy using the rgenetics rgFastQC wrapper - see http://rgenetics.org for details and licensing