Basic Statistics

Measure	Value
Filename	D1TB3ACXX_PR0055_02A15_H1_L007_R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	50140881
Filtered Sequences	0
Sequence length	100
%GC	42

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per base GC content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	199520	0.39791881598570233	No Hit
GGTAGTTCACTGTAAGATGACCTCGTTCATATCCTTTCCCATCTGTGTCC	107648	0.21469108211321616	No Hit
GATATCTCACTAAAGAGATAGCCGATCTTCCCAATAATCTTCACCAATAC	56585	0.1128520258748545	No Hit
GATTTCATAACGGAGAATAGAGGATTGAAGGAATCTCCACAATCAACAAA	51764	0.10323711703430181	No Hit

Kmer Content

Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	44893920	4.603159	9.396082	2
GGTGG	7636440	4.1987643	8.876761	75-79
AAAAA	33685255	4.0047812	15.362864	95-96
GTAGA	12217490	3.077795	5.6548786	65-69
TGTAG	12441590	3.0428424	5.5109844	60-64
GTCGG	6023890	2.6264553	5.7038784	45-49
CGGTG	5547925	2.4189312	6.189177	75-79
GTGGT	6598845	2.3842838	5.4463615	75-79
GGTCG	4643850	2.024749	5.8377094	80-84
CGCCG	4658255	1.9434872	5.8249826	80-84
AGGCA	4324605	1.3146408	6.3336577	1
GGTAG	3139310	1.1683644	6.0488625	1
AGGCT	3695365	1.090596	5.6976523	1

Files created by FastQC

D1TB3ACXX_PR0055_02A15_H1_L007_R2_fastqc.zip (176.0 KB)

duplication_levels.png (18.8 KB)

fastqc_data.txt (8.1 KB)

fastqc_report.html (8.5 KB)

kmer_profiles.png (65.7 KB)

per_base_gc_content.png (10.3 KB)

per_base_n_content.png (7.7 KB)

per_base_quality.png (9.9 KB)

per_base_sequence_content.png (18.2 KB)

per_sequence_gc_content.png (26.9 KB)

per_sequence_quality.png (19.6 KB)

rgFastQCOnPOyg.log (1.7 KB)

sequence_length_distribution.png (17.2 KB)

summary.txt (751 B)

FastQC documentation and full attribution is here

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FastQC was run by Galaxy using the rgenetics rgFastQC wrapper - see http://rgenetics.org for details and licensing